Phospho-ECT2 (Thr790)Rabbit anti-Canine, Chicken, Chimpanzee, Human, Mouse, Rat, Zebrafish, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue
• Reactivity occurs against human ECT2 protein at the pT790 residue
• By ELISA the antibody is specific for the phosphorylated form of the protein
• Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2
• Additional experiments are underway to clarify antibody specificity
• A BLAST analysis was used to suggest cross reactivity with ECT2 protein from human, mouse, rat, zebrafish, chimpanzee, chicken, and dog based on 100% homology with the immunizing sequence
• Reactivity against homologues from other sources is not known
• The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators
• The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases
• In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver
• Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.