Phospho-Cyclin B1 (Ser126)Rabbit anti-Canine, Chimpanzee, Hamster, Human, Mouse, Rat, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This antibody is specific for phosphorylated human Cyclin B1 protein at the pS126 residue
• BLAST analysis indicates 100 % homology of the immunizing sequence with Cyclin B1 from human
• Cross reactivity may occur with Cyclin B1 from rat, dog and chimpanzee as only a single amino acid change was detected within the immunogen sequence from these sources (91% homology)
• Partial reactivity may also occur with Cyclin B1 from mouse and hamster where two amino acids changes was noted (83% homology)
• Minimal reactivity is expected with the non-phosphorylated form of the protein
• Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs)
• Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis
• It is essential for the control of the cell cycle at the G2/M transition
• Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF)
• Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase
• Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis
• Destruction of Cyclin B1 is required for cell cycle progression
• Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.