Phospho-CREB (Ser133)Rabbit anti-Human, Mouse, Rat, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB
• Reactivity with non-phosphorylated CREB is minimal
• This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat
• CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors
• Activation of CREB is dependent upon the phosphorylation of serine 133
• Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1
• Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor
• CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133
• CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory
• In humans, the gene is located on the q arm of chromosome 2
• Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.