Aubergine/StingRabbit anti-Drosophila, Polyclonal, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• Anti-Aubergine affinity-purified antibody is directed against Drosophila Aubergine protein
• A BLAST analysis was used to suggest limited cross reactivity with Aubergine protein from other sources
• Aubergine (Sting) is a Drosophila protein involved in germ-line development and RNA interference
• Drosophila melanogaster has a robust and efficient innate immune system, which reacts to infections ranging from bacteria to fungi and, as discovered recently, viruses as well
• The known Drosophila immune responses rely on humoral and cellular activities, similar to those found in the innate immune system of other animals
• Recently, RNAi or 'RNA silencing' has arisen as a possible means by which Drosophila can react to specific pathogens, transposons and retroviral elements, in a fashion similar to that of a traditional mammalian adaptive immune system instead of in a more generalized and genome encoded innate immune-based response
• RNAi is a highly conserved regulation and defense mechanism, which suppresses gene expression via targeted RNA degradation directed by either exogenous dsRNA (cleaved into siRNAs) or endogenous miRNAs
• In plants, RNAi has been found to act as an antiviral immune response system
• In Drosophila multiple core RNAi pathway genes have been reported, including piwi, vasa intronic gene (vig), aubergine (aub), armitage (armi), Rm62, r2d2 and Argonaute2 (AGO2).