Phospho-C-Myb (Thr486) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue
• Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein.This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells
• Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot
• A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence
• Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence
• Cross-reactivity with c-Myb from other sources has not been determined
• Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'
• Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.