Methyl-STAT5 (Arg31) Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This antibody is specific for STAT5 R31/Me1 and does not reacts with other STATs surrounding aa31
• A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and rat
• Cross-reactivity with STAT5 from other sources has not been determined
• STAT5 alpha is a member of the STAT family of transcription factors
• In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators
• STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones
• Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis
• The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells
• STAT5 alpha, along with STAT5 beta, were identified in mouse
• The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice
• However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue
• STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.