MeCP2 Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity purified antibody is directed against human MeCP2
• A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to mouse, rat, and macaca fascicularis
• MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain
• MECP2 is capable of binding specifically to methylated DNA
• DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development
• MECP2 can also repress transcription from methylated gene promoters
• In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation
• MECP2 is dispensible in stem cells, but is essential for embryonic development
• MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.