CD74 Monoclonal Antibody (5-329), PE, eBioscience™, Invitrogen™

Description

• Description: This 5-329 monoclonal antibody recognizes human CD74, also known as the MHC Class II-associated invariant chain
• A 33-43 kDa nonpolymorphic type II membrane protein, CD74 is highly expressed on B cells and subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells
• Monocytes and macrophages also express CD74, but at lower levels
• CD74 acts as a chaperone of MHC Class II (HLA-DR) proteins, promoting their translocation from the ER to endocytic compartments during antigen presentation
• Activation of NF\kappaB and ERK has been shown following CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF)
• CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells
• CD74 has also been demonstrated to interact with CXCR2 and CXCR4
• Applications Reported: This 5-329 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 5-329 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0
• HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB)
• The alpha chain gene contains 5 exons
• Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail
• DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5
• Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities
• Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation
• HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes
• Three loci, DR, DQ and DP, encode the major expressed products of the human class II region
• The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.