EGR2 Monoclonal Antibody (erongr2), PE, eBioscience™, Invitrogen™

Description

• Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20)
• Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells
• Egr2 is predomitly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking
• Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin
• Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis
• The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells
• The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis
• Applications Reported: This erongr2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This erongr2 antibody has been tested by intracellular flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
• 00-5523) and protocol
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• The EGR2 gene provides instructions for making a protein called early growth response 2, which is part of the early growth response family of proteins
• These proteins bind to specific areas of DNA and help control the activity of particular genes
• On the basis of this action, the proteins are referred to as transcription factors
• The early growth response 2 protein activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells
• Myelin promotes the efficient transmission of nerve impulses
• If myelin is lost (demyelination) or its structure is disrupted, the transmission of nerve impulses is impaired
• Mutations in the EGR2 gene can cause two forms of Charcot-Marie-Tooth disease, type 1D or type 4E (sometimes called congenital hypomyelinating neuropathy) or a severe form of type 1D (sometimes called Dejerine-Sottas syndrome) that begins during infancy or early childhood.