EGR2 Monoclonal Antibody (erongr2), APC, eBioscience™, Invitrogen™

Description

• Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20)
• Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells
• Egr2 is predomitly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking
• Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin
• Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis
• The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells
• The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis
• Applications Reported: This erongr2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This erongr2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
• 00-5523)
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• EGR2 Monoclonal antibody specifically detects EGR2 in Mouse samples
• It is validated for Flow Cytometry.