Placental Alkaline Phosphatase Monoclonal Antibody (8B6), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 (or 130 with 65 kDa) enzyme expressed by placental syncytiotrophoblastoid cells
• This enzyme has slightly different enzyme kinetics from other phosphatases
• PLAP can be found anchored to the plasma membrane in addition to the cytoplasm
• Expression is found in several germ line cancers; ovarian and testicular
• Expression is also present in human embryonic stem cells and bone marrow-derived mesenchymal stromal cells
• This 8B6 antibody reacts with both the Regan and Nagao isozymes
• Applications Reported: This 8B6 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue, and ELISA
• Applications Tested: This 8B6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta with trypsin digestion
• This antibody can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Placental Alkaline Phosphatase plays an important role in the regulation of specific inflammatory disease processes
• There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney for this form of alkaline phosphatase have been well characterized
• Placental Alkaline Phosphatase reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation
• Placental Alkaline Phosphatase is useful in the identification of testicular germ cell tumors
• Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA)
• A proposed function of Placental Alkaline Phosphatase is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development
• Placental Alkaline Phosphatase has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects
• The character of hypophosphatasia can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.