CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• The RA3-6B2 MAb reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule
• CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells
• However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen
• CD45R (PTPRC) is a member of the protein tyrosine phosphatase (PTP) family
• PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation
• CD45 contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains
• CD45 is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling
• CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
• CD45 protein exists in multiple isoforms as a result of alternative splicing, and these isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains
• Further, these CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• In addition to its role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
• CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
• Diseases associated with CD45 dysfunction include Severe Combined Immunodeficiency (T-cell negative, B-Cell Negative) and Hepatitis C.