53-045-382

CD45.1 Monoclonal Antibody (A20), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 53-045-382-Each-of-1 In Stock ₹ 21,903.36

53-045-382 - Each of 1

₹ 21,903.36

In Stock

Quantity

1

Base Price: ₹ 21,903.36

GST (18%): ₹ 3,942.605

Total Price: ₹ 25,845.965

Antigen

CD45.1

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P06800

Gene Symbols

Ptprc

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

19264

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

A20

Conjugate

Alexa Fluor 488

Gene

Ptprc

Gene Alias

B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen

Host Species

Mouse

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

Description

  • Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains
  • The strains that express CD45.1 include SJL/J, DA, STS/A and RIII
  • CD45.1 is expressed by all leukocytes in these strains
  • Applications Reported: This A20 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This A20 antibody has been tested by flow cytometric analysis of mouse splenocytes
  • This may be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
  • CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains
  • These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
  • Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

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Fischer Scientific

53-045-382

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Antigen:
CD45.1

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P06800

Gene Symbols:
Ptprc

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
19264

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
A20

Conjugate:
Alexa Fluor 488

Gene:
Ptprc

Gene Alias:
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen

Host Species:
Mouse

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Mouse

Product Type:
Antibody

Isotype:
IgG2a κ

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MilliporeSigma™

53-046-30001

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Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
__

Regulatory Status:
__

Gene ID (Entrez):
__

Content And Storage:
Protect from light

Form:
Powder

Applications:
__

Clone:
__

Conjugate:
__

Gene:
__

Gene Alias:
__

Host Species:
__

Quantity:
10 mg

Primary or Secondary:
__

Target Species:
__

Product Type:
__

Isotype:
__

Img

Invitrogen

53-047-942

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Antigen:
CD47

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
Q08722

Gene Symbols:
CD47

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
961

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

Applications:
Flow Cytometry

Clone:
B6H12

Conjugate:
Alexa Fluor 488

Gene:
CD47

Gene Alias:
9130415E20Rik; AA407862; AI848868; antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; AW108519; B430305P08Rik; Cd47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen/integrin-associated protein; CD47 glycoprotein; CD47 molecule; CD47/IAP; IAP; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; Itgp; Leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

Host Species:
Mouse

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG1 κ

Img

MilliporeSigma™

53-048-60001

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Antigen:
__

Classification:
__

Concentration:
__

Formulation:
__

Gene Accession No.:
__

Gene Symbols:
__

Purification Method:
__

Regulatory Status:
__

Gene ID (Entrez):
__

Content And Storage:
Protect from light

Form:
Powder

Applications:
__

Clone:
__

Conjugate:
__

Gene:
__

Gene Alias:
__

Host Species:
__

Quantity:
10 mg

Primary or Secondary:
__

Target Species:
__

Product Type:
__

Isotype:
__