P-cadherin Monoclonal Antibody (12H6), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 12H6 recognizes human P-cadherin (placental-cadherin), a calcium dependent cell-cell adhesion protein
• This 118 kDa transmembrane protein was originally named due to its expression in mouse placental tissue
• Unfortunately, it is not expressed in human placental tissue
• The function of the molecule will depend on the associations with the specific member of the cytoplasmic catenin family (alpha, beta and gamma)
• The specific interaction defines the strength and signaling of the cell-cell interaction
• Expression is less broad than E-cadherin; P-cadherin is restricted to the basal proliferative cell layer of stratified epithelia
• During development expression is found in the proliferating tissues such as hair follicle keratinocytes and the growth regions in ductal mammary tissue
• There is evidence that P-cadherin can be secreted from epithelial cells during the late stages of pregcy
• P-cadherin expression is associated with poor prognosis for breast cancer while reduced expression in melanomas and squamous cell carcinomas may also result in poor prognosis
• Applications Reported: This 12H6 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P), and immunocytochemical staining (ICC)
• Applications Tested: This 12H6 antibody has been tested immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human skin with low pH antigen retrieval
• P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily
• Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin
• Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation
• P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium
• P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle
• Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas
• Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.