HES1 Monoclonal Antibody (4H1HES1), eBioscience™, Invitrogen™

Description

• Description: This 4H1HES monoclonal antibody reacts with human Hes1, a 30-kDa basic-helix-loop-helix transcriptional repressor
• Hes1 is expressed in thymocytes, as well as neural, retinal, liver, and pancreatic tissues
• Expression of Hes1 is controlled by Notch signaling, and thus plays an important role in T cell development
• This regulation by Notch is also critical for neural stem cell differentiation and neurogenesis
• Reports have demonstrated that Hes1 antagonizes transcription of MASH1, a basic-helix-loop-helix factor involved in neuronal determination
• Finally, Hes1 has been associated with Notch-induced T cell acute lymphoblastic lymphoma and other cancers
• Applications Reported: This 4H1HES antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (FFPE) tissue sections (IHC-P)
• Applications Tested: This 4H1HES antibody has been tested by immunohistochemistry on FFPE human kidney cortex at less than or equal to 20 μg/mL both without antigen retrieval or with high pH antigen retrieval
• It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• This protein belongs to the basic helix-loop-helix family of transcription factors
• It is a transcriptional repressor of genes that require a bHLH protein for their transcription
• The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.