CD208 (DC-LAMP) Monoclonal Antibody (31B), APC, eBioscience™, Invitrogen™

Description

• Description: This 31B monoclonal antibody reacts with human CD208, which is also known as dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP)
• CD208 is expressed almost exclusively by mature dendritic cells
• Expression of this molecule can be induced in monocytes by stimulation with GM-CSF and IL-4
• Moreover, CD208 can be upregulated in CD34+-derived dendritic cells by GM-CSF, SCF, and TNFa
• CD208 is also expressed in dendritic cells of non-lymphoid tissues such as skin
• While CD208 can be detected on the cell surface, this protein is mainly localized within lysosomes
• CD208 plays a key role in the processing and presentation of antigen during the immune response
• The 31B antibody only recognizes intracellular CD208, not the cell surface-expressed protein
• Applications Reported: This 31B antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 31B antibody has been pre-titrated and tested by flow cytometric analysis of in vitro differentiated mature dendritic cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Dendritic cells (DCs) are the most potent antigen-presenting cells
• Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).