GFAP Monoclonal Antibody (GA5), eFluor™ 615, eBioscience™, Invitrogen™

Description

• Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP)
• This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development
• Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predomit form expressed in astrocytes
• GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly
• Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain
• Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease
• Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors
• Applications Reported: This GA5 antibody has been reported for use in immunocytochemical (ICC) and immunohistochemical staining of frozen tissue sections (IHC-F)
• Applications Tested: This GA5 antibody has been tested by immunocytochemistry on fixed and permeabilized C6 cells at less than or equal to 10 μg/mL
• This product has not been validated for flow cytometric analysis
• Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75)
• GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family
• GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves
• In addition, neural stem cells strongly express GFAP
• Antibodies to GFAP are very useful as markers of astrocytic cells
• In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP
• GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes
• GFAP is used as a marker to distinguish astrocytes from other glial cells during development
• Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system
• Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.