TCR V beta 12 Monoclonal Antibody (MR11-1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This MR11-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 12 chain
• Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement
• The MR11-1 antibody recognizes the V beta 12 chain on T cells from mouse strains with the b haplotype, including C57BL/6, B10, and C58/J
• V beta 12+ T cells are absent in strains with the a (e.g., SJL) or c (e.g., RIII) haplotypes of the Tcrb gene complex
• Finally, mouse strains expressing the minor lymphocyte stimulatory (Mls) antigens, such as BALB/c and C3H, express fewer V beta 12+ T cells
• Applications Reported: This MR11-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MR11-1 antibody has been tested by flow cytometric analysis of mouse lymph node
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response
• TCR recognition of self-peptides has been linked to autoimmune disease
• Mutant self-peptides have been associated with tumors
• Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome
• Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE)
• The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5)
• T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP)
• A small number of T cells lack both CD4 and CD8 (double negative, DN)
• Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders
• Gamma-delta T cells are primarily found within the epithelium
• They show less TCR diversity and recognize antigens differently than alpha-beta T cells
• Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.