CXCL16 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHLP
• Preparation: Produced from sera of goats immunized with highly pure Recombinant Murine CXCL16
• Anti-Murine CXCL16-specific antibody was purified by affinity chromatography and then biotinylated
• Sandwich ELISA: To detect mCXCL16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
• This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine CXCL16 (500-P201G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mCXCL16
• Western Blot: To detect Murine CXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• 500-P201GBT-1MG will be provided as 2 x 500 μg CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor
• CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses
• The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines
• The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids
• The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence
• The extracellular domain contains 89 amino acid residues (86 a.a
• residues for the murine homolog).