TNF alpha Monoclonal Antibody (TN3-19.12), eFluor™ 660, eBioscience™, Invitrogen™

Description

• The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor alpha (TNF alpha), but not with human TNF alpha
• The TN3-19.12 antibody is a neutralizing antibody
• Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells
• A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule
• TNF alpha is cytolytic and plays an important role in immune regulation
• Dimers and trimers of TNF alpha have been observed
• Applications Reported: This TN3-19.12 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This TN3-19.12 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splecnoytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor 660 is a replacement for Alexa Fluor 647
• eFluor 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
• This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
• TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
• TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
• Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
• Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
• TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
• This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.