CD205 Monoclonal Antibody (MG38), FITC, eBioscience™, Invitrogen™

Description

• Description: The MG38 monoclonal antibody reacts with human CD205, also known as DEC-205, an integral membrane protein homologous to the macrophage mannose receptor
• CD205 is an endocytic dendritic receptor that mediates efficient processing and presentation of antigens on MHC class II productions in vivo
• CD205 is highly expressed by dendritic cells (DCs) within the T cell areas of lymphoid tissues, particularly on CD8^+ DCs that are believed to play a role in cross-priming
• Expression of CD205 is detected with this antibody at high intensity on in vitro derived mature human DCs (mDC) and at intermediate intensity on immature human DCs (iDC)
• All leukocytes from peripheral blood show a degree of staining with this mAb that can be minimized by using very low concentrations of MG38
• Applications Reported: The MG38 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This MG38 antibody has been tested by flow cytometric analysis of in vitro derived human dendritic cells and peripheral blood cells
• This can be used at less than or equal to 0.03 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity
• CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells
• CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region
• Further, CD205 also prompts the reduced proliferation of B lymphocytes
• Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.