IgE Monoclonal Antibody (23G3), FITC, eBioscience™, Invitrogen™

Description

• Description: The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE
• It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM
• Applications Reported: 23G3 has been reported for use in ELISA and flow cytometric analysis
• Applications Tested: This 23G3 antibody has been tested by ELISA and can be used at 1:5000-1:10000
• This 23G3 antibody has also been tested by flow cytometric analysis of IGEL cells, and can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• IgE is one of the five classes of immunoglobulins found in mammals
• Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains
• IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells
• Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations
• IgE is also functionally important for immunity against parasites.