IgM Monoclonal Antibody (SA-DA4), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM
• It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells
• Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific
• This variation can be attributed to both donor variability and the anticoagulant used during blood draw
• Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody
• Applications Reported: This SA-DA4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SA-DA4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• We recommend using a 450/50 bandpass filter, or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.