Leptin Polyclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
• Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Leptin
• Anti-Human Leptin-specific antibody was purified by affinity chromatography employing an immobilized Human Leptin matrix
• Sandwich ELISA: To detect Human Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
• This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Leptin (500-P86Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Leptin
• Western Blot: To detect Human Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
• Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Leptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
• Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function
• Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene
• Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body
• Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta
• Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells
• Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure
• Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors
• Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth
• Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension
• Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation
• Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.