5053843

Caspase-9 Antibody, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 19 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Caspase-9

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Rabbit

Research Discipline

Alzheimers Research, Apoptosis, Cancer, Caspases, Cell Biology, Neurodegeneration

Formulation

Whole antisera with 0.05% Sodium Azide

Gene Alias

APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c, ICE-like apoptotic protease 6, MCH6apoptosis-related cysteine protease

Gene Symbols

CASP9

Isotype

IgG

Purification Method

Unpurified

Test Specificity

This antibody detects both the pro- and active-Caspase 9 forms. In western blots, the proform of caspase-9 is detected at ∼50 kDa, the large subunit at ∼35 kDa, and the small subunit at ∼15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ∼21 kDa.

Dilution

Western Blot reported in scientific literature (PMID 24184144), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 22223358), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen Assay Dependent, Flow (Intracellular)

Classification

Polyclonal

Form

Antisera

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Canine, Gerbil

Gene Accession No.

P55211

Gene ID (Entrez)

842

Immunogen

Human Caspase 9 recombinant protein catalytic subunits (NP_001220).

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Description

  • Caspase-9 Polyclonal specifically detects Caspase-9 in Human, Mouse, Rat, Canine, Gerbil samples
  • It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).

Compare Similar Items

Show Difference

Img

Fischer Scientific

5053843

Rabbit Polyclonal Antibody has been...


Antigen:
Caspase-9

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Alzheimers Research, Apoptosis, Cancer, Caspases, Cell Biology, Neurodegeneration

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c, ICE-like apoptotic protease 6, MCH6apoptosis-related cysteine protease

Gene Symbols:
CASP9

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
This antibody detects both the pro- and active-Caspase 9 forms. In western blots, the proform of caspase-9 is detected at ∼50 kDa, the large subunit at ∼35 kDa, and the small subunit at ∼15 kDa. Intermediate caspase-9 cleavage forms may also be seen at ∼21 kDa.

Dilution:
Western Blot reported in scientific literature (PMID 24184144), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 22223358), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen Assay Dependent, Flow (Intracellular)

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Canine, Gerbil

Gene Accession No.:
P55211

Gene ID (Entrez):
842

Immunogen:
Human Caspase 9 recombinant protein catalytic subunits (NP_001220).

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Img

Fischer Scientific

5053846

Rabbit Polyclonal Antibody has been...


Antigen:
Caspase-9

Applications:
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Apoptosis, Cancer, Caspases, Cell Biology

Formulation:
50 ul neat whole antisera with 0.05% Sodium Azide

Gene Alias:
APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c, ICE-like apoptotic protease 6, MCH6apoptosis-related cysteine protease

Gene Symbols:
CASP9

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
This polyclonal antisera recognizes the proform of caspase-9 (∼50 kDa), and the large (∼35 kDa) and small (∼15 kDa) subunits of active/cleaved Caspase-9. Intermediate caspase-9 cleavage forms may also be seen at ∼21 kDa.

Dilution:
Western Blot 1:1000-1:2000, Simple Western 1:200, Immunohistochemistry, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Canine

Gene Accession No.:
P55211

Gene ID (Entrez):
842

Immunogen:
Recombinant mutated non-cleavable pro form of human Caspase 9 (NP_001220).

Primary or Secondary:
Primary

Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.

Img

Fischer Scientific

5053852

Rabbit Polyclonal Antibody...


Antigen:
Caspase-9

Applications:
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Apoptosis, Cancer, Caspases, Cell Biology

Formulation:
Whole antisera with 0.02% Sodium Azide

Gene Alias:
APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c, ICE-like apoptotic protease 6, MCH6apoptosis-related cysteine protease

Gene Symbols:
CASP9

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
Caspase-9

Dilution:
Western Blot 1:1000-1:2000, Immunohistochemistry 1:200-1:500, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:500

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
842

Immunogen:
A synthetic peptide corresponding to amino acids 188-206 (EKLRRRFSSLHFMVEVKGD) of human caspase-9 was used as immunogen; GenBank no. gi 27802691 gb AAO21133.1 .

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Img

Fischer Scientific

5053855

Rabbit Polyclonal Antibody...


Antigen:
Caspase-9 (p Ser196)

Applications:
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Rabbit

Research Discipline:
Apoptosis, Cancer, Caspases, Cell Biology

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c, ICE-like apoptotic protease 6, MCH6apoptosis-related cysteine protease

Gene Symbols:
CASP9

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
Caspase-9

Dilution:
Western Blot 1:1000-1:2000, Immunohistochemistry 1:200 - 1:500, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:200 - 1:500

Classification:
Polyclonal

Form:
Antisera

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
842

Immunogen:
A synthetic phosphopeptide corresponding to amino acids 188-206 (EKLRRRFSS*LHFMVEVKGD) of human caspase-9 was used as immunogen; GenBank no. gi 27802691 gb AAO21133.1 . S* refers to phosphorylated serine.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.