Caspase-3 Antibody - (active/cleaved), Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 48 publications
Manufacturer: Fischer Scientific
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Antigen
Caspase-3
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Apoptosis, Cancer, Caspases, Phospho Specific
Formulation
Neat whole antisera with 0.05% Sodium Azide
Gene ID (Entrez)
836
Immunogen
This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
Primary or Secondary
Primary
Content And Storage
Store at -20C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
31.7 kDa
Dilution
Western Blot 1:1000-1:2000, Flow Cytometry reported in scientific literature (PMID 28287161), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 29963272), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441), Immunoblotting reported in scientific literature (PMID 27912032), Flow (Intracellular) reported in scientific literature (PMID 24804954)
Classification
Polyclonal
Form
Antisera
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Gerbil
Gene Alias
Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
Gene Symbols
CASP3
Isotype
IgG
Purification Method
Unpurified
Test Specificity
Preferentially detects active caspase-3 (large subunit: ∼14-21 kDa, and small subunit: ∼10 kDa). However, it may also detect pro-caspase-3 (∼32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.
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Description
- Caspase-3 Polyclonal specifically detects Caspase-3 in Human, Mouse, Rat, Gerbil samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Flow (Intracellular).
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Antigen: Caspase-3
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Apoptosis, Cancer, Caspases, Phospho Specific
Formulation: Neat whole antisera with 0.05% Sodium Azide
Gene ID (Entrez): 836
Immunogen: This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
Primary or Secondary: Primary
Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 31.7 kDa
Dilution: Western Blot 1:1000-1:2000, Flow Cytometry reported in scientific literature (PMID 28287161), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 29963272), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441), Immunoblotting reported in scientific literature (PMID 27912032), Flow (Intracellular) reported in scientific literature (PMID 24804954)
Classification: Polyclonal
Form: Antisera
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Gerbil
Gene Alias: Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
Gene Symbols: CASP3
Isotype: IgG
Purification Method: Unpurified
Test Specificity: Preferentially detects active caspase-3 (large subunit: ∼14-21 kDa, and small subunit: ∼10 kDa). However, it may also detect pro-caspase-3 (∼32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.
Antigen: Caspase-8
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Rabbit
Research Discipline: Apoptosis, Apoptosis Detections, Cancer, Caspases, Cell Biology, Phospho Specific, Ubiquitin Proteasome Pathway
Formulation: Whole antisera with 0.05% Sodium Azide
Gene ID (Entrez): 841
Immunogen: Recombinant catalytically active human Caspase-8 protein.
Primary or Secondary: Primary
Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 55.4 kDa
Dilution: Western Blot 1:1000-1:2000, Simple Western reported by internal validation, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen 1:10-1:500
Classification: Polyclonal
Form: Antisera
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Gerbil
Gene Alias: AIS, androgen receptor, DHTRTFM, Dihydrotestosterone receptorHYSP1, HUMARA, NR3C4KD, Nuclear receptor subfamily 3 group C member 4, SMAX1SBMA, spinal and bulbar muscular atrophy
Gene Symbols: CASP8
Isotype: IgG
Purification Method: Unpurified
Test Specificity: Detects Caspase-8 and cleavage products.
Antigen: AHR
Applications: Western Blot, ChIP Assay, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Goat
Research Discipline: Apoptosis, Breast Cancer, Cancer, Chromatin Research, Hypoxia, Transcription Factors and Regulators
Formulation: Whole antisera with No Preservative
Gene ID (Entrez): 196
Immunogen: N-terminal sequence of Aryl hydrocarbon Receptor purified from C57BL/6J mice. [UniProt# P30561]
Primary or Secondary: Primary
Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Dilution: Western Blot reported in scientific literature (PMID 31351871; 24302727; 3999131), Chromatin Immunoprecipitation reported in scientific literature (PMID 3999131), Immunohistochemistry 1:100 - 1:200, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry-Paraffin 1:100 - 1:200, Gel Super Shift Assays
Classification: Polyclonal
Form: Antisera
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Gene Alias: Ah receptor, AhR, AH-receptor, aryl hydrocarbon receptor, BHLHE76, bHLHe76aromatic hydrocarbon receptor, Class E basic helix-loop-helix protein 76
Gene Symbols: AHR
Isotype: __
Purification Method: Unpurified
Test Specificity: Human and mouse. Rat reactivity reported in the scientific literature (PMID: 18515748).
