5057769

Capicua Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 2 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Capicua

Dilution

Western Blot 1:1000-1:2500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 2-5 ug/mg lysate, Knockout Validated

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Cancer, Neuroscience

Formulation

PBS with 0.02% Sodium Azide

Gene Alias

capicua homolog (Drosophila), KIAA0306capicua (Drosophila) homolog, protein capicua homolog

Gene Symbols

CIC

Isotype

IgG

Purification Method

Affinity Purified

Molecular Weight of Antigen

250 kDa

Concentration

1 mg/mL

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, KnockDown

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Canine

Gene Accession No.

Q96RK0

Gene ID (Entrez)

23152

Immunogen

The epitope recognized by this antibody maps to a C-terminal region within residue 1500-1608 of human Capicua. [NP_055940.3]

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Description

  • Capicua Polyclonal specifically detects Capicua in Human, Mouse, Rat, Canine samples
  • It is validated for Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Knockout Validated.

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Antigen:
Capicua

Dilution:
Western Blot 1:1000-1:2500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 2-5 ug/mg lysate, Knockout Validated

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Neuroscience

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
capicua homolog (Drosophila), KIAA0306capicua (Drosophila) homolog, protein capicua homolog

Gene Symbols:
CIC

Isotype:
IgG

Purification Method:
Affinity Purified

Molecular Weight of Antigen:
250 kDa

Concentration:
1 mg/mL

Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, KnockDown

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Canine

Gene Accession No.:
Q96RK0

Gene ID (Entrez):
23152

Immunogen:
The epitope recognized by this antibody maps to a C-terminal region within residue 1500-1608 of human Capicua. [NP_055940.3]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Antigen:
Carbonic Anhydrase IX/CA9

Dilution:
Western Blot 1 - 3 ug/ml, Simple Western 1:50, Flow Cytometry 1:1000, ELISA reported in scientific literature (PMID 19963243), Immunohistochemistry 1:200 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml, Immunoprecipitation 1:10 - 1:500. Use reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:200 - 1:500, Immunohistochemistry-Frozen 1:200 - 1:500, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray reported in scientific literature (PMID 31955345), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Dual RNAscope ISH-IHC 1:1000

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Cellular Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Signal Transduction

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
CA-IX, CAIXcarbonic anhydrase 9, Carbonate dehydratase IX, carbonic anhydrase IXpMW1, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MNRCC-associated antigen G250, P54/58N, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250

Gene Symbols:
CA9

Isotype:
IgG

Purification Method:
Affinity Purified

Molecular Weight of Antigen:
55 kDa

Concentration:
1.0 mg/mL

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In situ PLA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Canine, Plant

Gene Accession No.:
Q16790

Gene ID (Entrez):
768

Immunogen:
A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]

Primary or Secondary:
Primary

Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.

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5058079

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Antigen:
Collagen I alpha 1

Dilution:
Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 10-20 ug/ml, Immunohistochemistry-Paraffin 1:200, Western Blot (Negative)

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cell Biology, Cellular Markers, Extracellular Matrix, Signal Transduction, Stem Cells

Formulation:
PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias:
Alpha-1 type I collagen, COL-IA1, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, Collagen Type I Alpha 1 Chain, collagen, type I, alpha 1, Collagen1, Collagen-1, EDSARTH1, OI4, pro-alpha-1 collagen type 1, Type I Procollagen Alpha 1 Chain

Gene Symbols:
COL1A1

Isotype:
IgG

Purification Method:
Affinity Purified

Molecular Weight of Antigen:
139 kDa

Concentration:
1.0 mg/ml

Applications:
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P02452

Gene ID (Entrez):
1277

Immunogen:
This Collagen I alpha 1 antibody was raised against a synthetic peptide made to an internal portion of the human Collagen I protein (between residues 150-200) [UniProt# P02452]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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5058095

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Antigen:
GFP

Dilution:
Western Blot 1:1000-1:10000, Flow Cytometry 1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Proximity Ligation Assay, Electron Microscopy 1:10 - 1:500, Immunohistochemistry Free-Floating 1:10 - 1:500, Knockout Validated, Knockdown Validated

Classification:
Polyclonal

Host Species:
Goat

Research Discipline:
__

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Alias:
Enhanced Green Fluorescent Protein, Green Fluorescent Protein, green fluorescent protein (gfp)

Gene Symbols:
GFP

Isotype:
IgG

Purification Method:
Affinity Purified

Molecular Weight of Antigen:
__

Concentration:
1.1 mg/ml

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Non-species specific

Gene Accession No.:
P42212

Gene ID (Entrez):
__

Immunogen:
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. (Uniprot: P42212)

Primary or Secondary:
Primary

Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.