Antigen:
Carbonic Anhydrase IX/CA9
Dilution:
Western Blot 1 - 3 ug/ml, Simple Western 1:50, Flow Cytometry 1:1000, ELISA reported in scientific literature (PMID 19963243), Immunohistochemistry 1:200 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml, Immunoprecipitation 1:10 - 1:500. Use reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:200 - 1:500, Immunohistochemistry-Frozen 1:200 - 1:500, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray reported in scientific literature (PMID 31955345), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Dual RNAscope ISH-IHC 1:1000
Classification:
Polyclonal
Research Discipline:
Cancer, Cellular Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Signal Transduction
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
CA-IX, CAIXcarbonic anhydrase 9, Carbonate dehydratase IX, carbonic anhydrase IXpMW1, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MNRCC-associated antigen G250, P54/58N, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250
Purification Method:
Affinity Purified
Molecular Weight of Antigen:
55 kDa
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In situ PLA
Target Species:
Human, Mouse, Rat, Canine, Plant
Gene Accession No.:
Q16790
Immunogen:
A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]
Primary or Secondary:
Primary
Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.