5057989

Carbonic Anhydrase IX/CA9 Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Carbonic Anhydrase IX/CA9

Dilution

Western Blot 1 - 3 ug/ml, Simple Western 1:50, Flow Cytometry 1:1000, ELISA reported in scientific literature (PMID 19963243), Immunohistochemistry 1:200 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml, Immunoprecipitation 1:10 - 1:500. Use reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:200 - 1:500, Immunohistochemistry-Frozen 1:200 - 1:500, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray reported in scientific literature (PMID 31955345), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Dual RNAscope ISH-IHC 1:1000

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Cancer, Cellular Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Signal Transduction

Formulation

PBS with 0.02% Sodium Azide

Gene Alias

CA-IX, CAIXcarbonic anhydrase 9, Carbonate dehydratase IX, carbonic anhydrase IXpMW1, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MNRCC-associated antigen G250, P54/58N, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250

Gene Symbols

CA9

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

Human, dog, rat and mouse (PMID 22842475).

Concentration

1.0 mg/mL

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In situ PLA

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Canine, Plant

Gene Accession No.

Q16790

Gene ID (Entrez)

768

Immunogen

A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]

Primary or Secondary

Primary

Content And Storage

Store at -20C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

55 kDa

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Description

  • Carbonic Anhydrase IX/CA9 Polyclonal specifically detects Carbonic Anhydrase IX/CA9 in Human, Mouse, Rat, Plant samples
  • It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray, Dual RNAscope ISH-IHC.

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Antigen:
Carbonic Anhydrase IX/CA9

Dilution:
Western Blot 1 - 3 ug/ml, Simple Western 1:50, Flow Cytometry 1:1000, ELISA reported in scientific literature (PMID 19963243), Immunohistochemistry 1:200 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml, Immunoprecipitation 1:10 - 1:500. Use reported in multiple pieces of scientific literature, Immunohistochemistry-Paraffin 1:200 - 1:500, Immunohistochemistry-Frozen 1:200 - 1:500, Immunoblotting, Gel Super Shift Assays, Proximity Ligation Assay, Microarray reported in scientific literature (PMID 31955345), Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Dual RNAscope ISH-IHC 1:1000

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Cellular Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Signal Transduction

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
CA-IX, CAIXcarbonic anhydrase 9, Carbonate dehydratase IX, carbonic anhydrase IXpMW1, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MNRCC-associated antigen G250, P54/58N, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250

Gene Symbols:
CA9

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, dog, rat and mouse (PMID 22842475).

Concentration:
1.0 mg/mL

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In situ PLA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Canine, Plant

Gene Accession No.:
Q16790

Gene ID (Entrez):
768

Immunogen:
A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]

Primary or Secondary:
Primary

Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
55 kDa

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Antigen:
Collagen I alpha 1

Dilution:
Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 10-20 ug/ml, Immunohistochemistry-Paraffin 1:200, Western Blot (Negative)

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cell Biology, Cellular Markers, Extracellular Matrix, Signal Transduction, Stem Cells

Formulation:
PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias:
Alpha-1 type I collagen, COL-IA1, Collagen 1, Collagen 1 alpha 1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen of skin, tendon and bone, alpha-1 chain, Collagen Type I Alpha 1 Chain, collagen, type I, alpha 1, Collagen1, Collagen-1, EDSARTH1, OI4, pro-alpha-1 collagen type 1, Type I Procollagen Alpha 1 Chain

Gene Symbols:
COL1A1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Mouse. Predicted reactivity in Human.

Concentration:
1.0 mg/ml

Applications:
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P02452

Gene ID (Entrez):
1277

Immunogen:
This Collagen I alpha 1 antibody was raised against a synthetic peptide made to an internal portion of the human Collagen I protein (between residues 150-200) [UniProt# P02452]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
139 kDa

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Antigen:
GFP

Dilution:
Western Blot 1:1000-1:10000, Flow Cytometry 1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Proximity Ligation Assay, Electron Microscopy 1:10 - 1:500, Immunohistochemistry Free-Floating 1:10 - 1:500, Knockout Validated, Knockdown Validated

Classification:
Polyclonal

Host Species:
Goat

Research Discipline:
__

Formulation:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide

Gene Alias:
Enhanced Green Fluorescent Protein, Green Fluorescent Protein, green fluorescent protein (gfp)

Gene Symbols:
GFP

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
No reaction was observed against Human, Mouse or Rat serum proteins.

Concentration:
1.1 mg/ml

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Non-species specific

Gene Accession No.:
P42212

Gene ID (Entrez):
__

Immunogen:
The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. (Uniprot: P42212)

Primary or Secondary:
Primary

Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

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Antigen:
CARD12

Dilution:
Western Blot 1:1000 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunoprecipitation 1:50 - 1:200, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Immunohistochemistry-Frozen 1:10 - 1:500

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Apoptosis, Immunology, Innate Immunity

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
CARD12leucine rich repeat and CARD domaincontaining 4, caspase recruitment domain family, member 12, Caspase recruitment domain-containing protein 12, Clan protein, CLAN1ipaf, CLANCARD, LRR, and NACHT-containing protein, CLR2.1, Ice protease-activating factor, Ipaf, NLR family, CARD domain containing 4

Gene Symbols:
NLRC4

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
Ipaf

Concentration:
__

Applications:
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
Q9NPP4

Gene ID (Entrez):
58484

Immunogen:
A synthetic peptide corresponding to within amino acis 610 and 660 of Ipaf/Clan/CARD12 was used as immunogen, from genbank sequence NP_067032.3

Primary or Secondary:
Primary

Content And Storage:
Store at -20C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__