HIF-1 alpha Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 175 publications
Manufacturer: Fischer Scientific
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Antigen
HIF-1 alpha
Dilution
Western Blot 1:500 - 1:1000, Simple Western 1:100, ELISA, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Immunoblotting, Gel Super Shift Assays 1:1 - 1:100, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635), Dual RNAscope ISH-IHC
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
Formulation
PBS with 0.02% Sodium Azide
Gene Alias
AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
Gene Symbols
HIF1A
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
Human, monkey, mouse, rat and Xenopus. Canine reactivity reported in scientific literature (PMID: 24140705) Mouse results are mixed.
Concentration
1.0 mg/mL
Applications
ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Gel Shift, ChIP Assay, Immunoassay
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus species, Zebrafish
Gene Accession No.
Q61221, O35800
Gene ID (Entrez)
3091
Immunogen
This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
93 kDa
Related Products
Description
- HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus, Zebrafish samples
- It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Gel Super Shift Assays, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing, Dual RNAscope ISH-IHC.
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Antigen: HIF-1 alpha
Dilution: Western Blot 1:500 - 1:1000, Simple Western 1:100, ELISA, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Immunoblotting, Gel Super Shift Assays 1:1 - 1:100, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635), Dual RNAscope ISH-IHC
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
Gene Symbols: HIF1A
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: Human, monkey, mouse, rat and Xenopus. Canine reactivity reported in scientific literature (PMID: 24140705) Mouse results are mixed.
Concentration: 1.0 mg/mL
Applications: ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Gel Shift, ChIP Assay, Immunoassay
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus species, Zebrafish
Gene Accession No.: Q61221, O35800
Gene ID (Entrez): 3091
Immunogen: This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: 93 kDa
Antigen: FLIP
Dilution: Western Blot 1:1000-1:2000, Immunohistochemistry, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: __
Formulation: Whole antisera with 0.05% Sodium Azide
Gene Alias: CASHFADD-like antiapoptotic molecule 1, CASP8 and FADD-like apoptosis regulator, CASP8AP1, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, caspase-related inducer of apoptosis, CASPER, CasperFLAME1, Cellular FLICE-like inhibitory protein, c-FLIPR, c-FLIPS, CLARPMACH-related inducer of toxicity, FADD-like apoptotic molecule, FLAME, FLAME-1, I-FLICEMRITc-FLIPFLIPc-FLIPL, Inhibitor of FLICE, Usurpin, usurpin beta
Gene Symbols: CFLAR
Isotype: IgG
Purification Method: Unpurified
Test Specificity: The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms.
Concentration: __
Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: __
Gene ID (Entrez): 8837
Immunogen: A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP was used as immunogen, GenBank no. gi 12643547. This amino acid sequnce is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www.ncbi.nlm.nih.gov/blast/.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Antigen: FMNL2
Dilution: Western Blot 1-3 ug/ml, Immunohistochemistry-Paraffin 5-10 ug/ml
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: __
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: FHOD2, FLJ37546, formin homology 2 domain containing 2, Formin homology 2 domain-containing protein 2, formin-like 2, formin-like protein 2, KIAA1902
Gene Symbols: FMNL2
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: The amino acid sequence used as immunogen is 100% homologous in human, chimpanzee, monkey, rat and mouse, 94% homologous in cow, 92% homologous in chicken and 88% homologous in opossum.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Primate
Gene Accession No.: Q9Y613
Gene ID (Entrez): 114793
Immunogen: A portion of amino acid 1-50 of human Formin-like protein 2 was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
