5078398

LC3B Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 1218 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

LC3B

Dilution

Western Blot 0.5 - 2.0 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200 - 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 ug/500 ug of protein, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience

Formulation

PBS with 0.02% Sodium Azide

Gene Alias

anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B

Gene Symbols

MAP1LC3B

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.

Concentration

1.0 mg/mL

Applications

ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, KnockDown

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish

Gene Accession No.

Q9GZQ8

Gene ID (Entrez)

81631

Immunogen

Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]

Primary or Secondary

Primary

Content And Storage

Store at -20°C.

Molecular Weight of Antigen

14.688 kDa

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Description

  • LC3B Polyclonal specifically detects LC3B in Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Chinese Hamster, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated.

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Antigen:
LC3B

Dilution:
Western Blot 0.5 - 2.0 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200 - 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 ug/500 ug of protein, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B

Gene Symbols:
MAP1LC3B

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.

Concentration:
1.0 mg/mL

Applications:
ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, KnockDown

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish

Gene Accession No.:
Q9GZQ8

Gene ID (Entrez):
81631

Immunogen:
Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]

Primary or Secondary:
Primary

Content And Storage:
Store at -20°C.

Molecular Weight of Antigen:
14.688 kDa

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Antigen:
LCAT

Dilution:
Western Blot 1:1000, ELISA 1:500

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Cholesterol Metabolism, Lipid and Metabolism, Signal Transduction

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
EC 2.3.1.43, lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase

Gene Symbols:
LCAT

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
__

Concentration:
__

Applications:
Western Blot, ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P04180

Gene ID (Entrez):
3931

Immunogen:
Carboxyl-terminal histidine-tagged human recombinant LCAT purified from the medium of CHO cells overexpressing human LCAT cDNA. [UniProt# P04180]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

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5078513

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Antigen:
LCAT

Dilution:
Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:500

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Cholesterol Metabolism, Lipid and Metabolism, Signal Transduction

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
EC 2.3.1.43, lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase

Gene Symbols:
LCAT

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
__

Concentration:
__

Applications:
Western Blot, Immunocytochemistry, Immunofluorescence

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P04180

Gene ID (Entrez):
3931

Immunogen:
Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

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5078634

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Antigen:
Porimin

Dilution:
Western Blot 1-3 ug/ml, Immunohistochemistry, Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (PMID 16814713)

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Apoptosis

Formulation:
PBS containing 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
KCT-3, KCT3keratinocytes associated transmembrane protein 3, Keratinocytes-associated transmembrane protein 3, PORIMINporimin, pro oncosis receptor inducing membrane injury, Pro-oncosis receptor inducing membrane injury, serine/threonine-rich receptor, transmembrane protein 123PORMIN

Gene Symbols:
TMEM123

Isotype:
IgG

Purification Method:
Protein G purified

Test Specificity:
Reacts with Human and bovine. This sequence is 100% conserved in cow, and 93% conserved in rat, mouse, and chicken.

Concentration:
0.5 mg/mL

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Bovine

Gene Accession No.:
Q8N131

Gene ID (Entrez):
114908

Immunogen:
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 173-188 SRRGIRYRTIDEHDAI of human Porimin; GenBank no. AAG2164.1.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__