ATG5 Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 211 publications
Manufacturer: Fischer Scientific
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Antigen
ATG5
Dilution
Western Blot 1:500, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:250, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:400, Immunoblotting, Radioimmunoassay, Proximity Ligation Assay, Electron Microscopy, Knockout Validated, KnockDown Validated
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Apoptosis, Autophagy, Cancer, Cell Biology, Ubiquitin Proteasome Pathway
Formulation
PBS with 0.02% Sodium Azide
Gene Alias
APG5 autophagy 5-like (S. cerevisiae), ASPAPG5-LIKE, ATG5 autophagy related 5 homolog (S. cerevisiae), Autophagy protein 5
Gene Symbols
ATG5
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.
Concentration
1.0 mg/ml
Applications
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoblot, Radioimmune Assays (RIA), In situ PLA, Immunoassay, KnockDown
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Alligator, Bovine, Drosophila, Fish, Guinea Pig, Primate, Xenopus species, Zebrafish
Gene Accession No.
Q9H1Y0
Gene ID (Entrez)
9474
Immunogen
A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Description
- ATG5 Polyclonal specifically detects ATG5 in Human, Mouse, Rat, Porcine, Alligator, Bovine, Drosophila, Fish, Guinea Pig, Primate, Xenopus, Zebrafish samples
- It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunoblotting, Radioimmunoassay, Proximity Ligation Assay, Electron Microscopy, Knockout Validated, Knockdown Validated.
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Antigen: ATG5
Dilution: Western Blot 1:500, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:250, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:400, Immunoblotting, Radioimmunoassay, Proximity Ligation Assay, Electron Microscopy, Knockout Validated, KnockDown Validated
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Apoptosis, Autophagy, Cancer, Cell Biology, Ubiquitin Proteasome Pathway
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: APG5 autophagy 5-like (S. cerevisiae), ASPAPG5-LIKE, ATG5 autophagy related 5 homolog (S. cerevisiae), Autophagy protein 5
Gene Symbols: ATG5
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing amino acids 1-79. The calpain cleaved form of ATG5 is missing amino acids 195-275.
Concentration: 1.0 mg/ml
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoblot, Radioimmune Assays (RIA), In situ PLA, Immunoassay, KnockDown
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Alligator, Bovine, Drosophila, Fish, Guinea Pig, Primate, Xenopus species, Zebrafish
Gene Accession No.: Q9H1Y0
Gene ID (Entrez): 9474
Immunogen: A synthetic peptide made to an N-terminal region of the human ATG5 protein (within residues 1-50) [Swiss-Prot Q9H1Y0]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: ATG5
Dilution: Western Blot 0.5 - 2 ug/ml, Immunohistochemistry 5 ug/ml, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Apoptosis, Autophagy, Cancer, Cell Biology, Ubiquitin Proteasome Pathway
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: APG5 autophagy 5-like (S. cerevisiae), ASPAPG5-LIKE, ATG5 autophagy related 5 homolog (S. cerevisiae), Autophagy protein 5
Gene Symbols: ATG5
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: The amino acid sequence used as immunogen is 100% homologous in human, opossum, horse, cow, dog, monkey, mouse, rat and chimpanzee and 95% homologous in chicken, 80% homologous in xenopus and 75% homologous in zebra fish.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine
Gene Accession No.: __
Gene ID (Entrez): 9474
Immunogen: A portion of amino acid 1-50 of human APG5L was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: __
Dilution: __
Classification: __
Host Species: __
Research Discipline: __
Formulation: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Test Specificity: __
Concentration: __
Applications: __
Conjugate: __
Regulatory Status: __
Target Species: __
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
