5067470

CD11b Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 67 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD11b

Dilution

Western Blot 2 ug/mL. Use reported in scientific literature (PMID 31082627), Simple Western 1:50, Flow Cytometry reported in scientific literature (PMID 21422470), Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200. Use reported in multiple pieces of scientific literature (PMID 23980916), Immunohistochemistry-Paraffin 1:400. Use reported in scientific literature (PMID 31022918), Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916), In-situ Hybridization reported in scientific literature (PMID 27133471), Flow (Cell Surface) 1:10 - 1:1000, Single Cell Western 1:10, Dual RNAscope ISH-IHC

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Breast Cancer, Cancer, Cellular Markers, Cytoskeleton Markers, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Microglia Markers, Myeloid Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation

PBS with 0.02% Sodium Azide

Gene Alias

antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, Cell surface glycoprotein MAC-1 subunit alpha, Complement component 3 receptor 3 subunit, CR-3 alpha chain, CR3A, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement

Gene Symbols

ITGAM

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

Mouse, rat and human. We have feedback that this antibody does not work in human samples with Western blot. Immunogen has 100% homology to bovine and 88% homology with sheep.

Concentration

1.00 mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), In Situ Hybridization (ISH), Flow Cytometry, In Situ Hybridization (ISH)

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque

Gene Accession No.

P11215, P05555

Gene ID (Entrez)

3684

Immunogen

Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]

Primary or Secondary

Primary

Content And Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

127.2 kDa

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Description

  • CD11b Polyclonal specifically detects CD11b in Human, Mouse, Rat, Bovine, C
  • elegans, Chinese Hamster, Monkey, Rhesus Macaque samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, In-situ Hybridization, Single Cell Western, Dual RNAscope ISH-IHC.

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Antigen:
CD11b

Dilution:
Western Blot 2 ug/mL. Use reported in scientific literature (PMID 31082627), Simple Western 1:50, Flow Cytometry reported in scientific literature (PMID 21422470), Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200. Use reported in multiple pieces of scientific literature (PMID 23980916), Immunohistochemistry-Paraffin 1:400. Use reported in scientific literature (PMID 31022918), Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916), In-situ Hybridization reported in scientific literature (PMID 27133471), Flow (Cell Surface) 1:10 - 1:1000, Single Cell Western 1:10, Dual RNAscope ISH-IHC

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Breast Cancer, Cancer, Cellular Markers, Cytoskeleton Markers, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Innate Immunity, Microglia Markers, Myeloid Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
antigen CD11b (p170), CD11 antigen-like family member B, CD11b, CD11b antigen, Cell surface glycoprotein MAC-1 subunit alpha, Complement component 3 receptor 3 subunit, CR-3 alpha chain, CR3A, CR3AMGC117044, integrin alpha-M, integrin, alpha M (complement

Gene Symbols:
ITGAM

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Mouse, rat and human. We have feedback that this antibody does not work in human samples with Western blot. Immunogen has 100% homology to bovine and 88% homology with sheep.

Concentration:
1.00 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), In Situ Hybridization (ISH), Flow Cytometry, In Situ Hybridization (ISH)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Bovine, C. elegans, Chinese Hamster, Monkey, Rhesus Macaque

Gene Accession No.:
P11215, P05555

Gene ID (Entrez):
3684

Immunogen:
Rabbit Polyclonal CD11b Antibody was made to a synthetic peptide within residues 250-350 of the mouse CD11b protein. [Swiss-Prot# P05555]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
127.2 kDa

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Antigen:
eIF4A2

Dilution:
Western Blot 0.5-1.0 ug/ml, Immunohistochemistry-Paraffin 5 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, mTOR Pathway

Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
ATP-dependent RNA helicase eIF4A-2, BM-010, DDX2Beukaryotic translation initiation factor 4A, isoform 2, EC 3.6.1, EC 3.6.4.13, EIF4A, eIF-4A-II, eIF4A-II, EIF4F, eukaryotic initiation factor 4A-II, eukaryotic translation initiation factor 4A, eukaryotic translation initiation factor 4A2

Gene Symbols:
EIF4A2

Isotype:
IgG

Purification Method:
Protein A purified

Test Specificity:
Human and mouse. 100% homologous in rat, bovine, and porcine.

Concentration:
0.5 mg/mL

Applications:
Western Blot, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
Q14240

Gene ID (Entrez):
1974

Immunogen:
A portion of amino acids 1-50 of human eIF4A2 was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

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Antigen:
eIF4B

Dilution:
Western Blot 0.25-0.50 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Core ESC Like Genes, mTOR Pathway, Stem Cell Markers

Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
EIF-4B, eukaryotic translation initiation factor 4B, PRO1843

Gene Symbols:
EIF4B

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey and chimpanzee, 83% homologous in dog and 72% homologous in rat and mouse.

Concentration:
0.5 mg/mL

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Bovine, Chicken, Primate

Gene Accession No.:
P23588

Gene ID (Entrez):
1975

Immunogen:
A portion of amino acids 150-200 of human eIF4B was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__

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5067492

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Antigen:
eIF4B

Dilution:
Western Blot 0.005-1.0 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Core ESC Like Genes, mTOR Pathway, Stem Cell Markers

Formulation:
0.2 ml PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
EIF-4B, eukaryotic translation initiation factor 4B, PRO1843

Gene Symbols:
EIF4B

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
The amino acid sequence used as immunogen is 100% homologous in human, rhesus monkey, chimpanzee, mouse, horse, opossum and Xenopus.

Concentration:
0.5 mg/mL

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Primate

Gene Accession No.:
P23588

Gene ID (Entrez):
1975

Immunogen:
A portion of amino acids 570-611 of human eIF4B was used as the immunogen.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen:
__