5073397

Histone H4, Trimethyl Lys20 Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Histone H4 (Trimethyl Lys20)

Dilution

Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry reported in scientific literature (PMID 23924899), Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 23924899), Dot Blot 0.5 ug/ml

Classification

Polyclonal

Host Species

Rabbit

Target Species

Human, Mouse, Rat, Mammalia

Gene Accession No.

P62805, P62806

Gene ID (Entrez)

121504

Immunogen

Synthetic trimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot# P62805].

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration

0.58 mg/mL

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Dot Blot

Conjugate

Unconjugated

Regulatory Status

RUO

Formulation

PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias

H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4/p, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, H4K20Me3, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, histone 4, H4, histone cluster 4, H4, histone H4, MGC24116

Gene Symbols

HIST4H4

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

Human, mouse and rat. Predicted to react with most mammal species.

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Description

  • Description Histone H4 Polyclonal specifically detects Histone H4 in Human, Mouse, Rat, Mammal samples
  • It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Dot Blot.

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Fischer Scientific

5073397

Rabbit Polyclonal Antibody has been...


Antigen:
Histone H4 (Trimethyl Lys20)

Dilution:
Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry reported in scientific literature (PMID 23924899), Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 23924899), Dot Blot 0.5 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Target Species:
Human, Mouse, Rat, Mammalia

Gene Accession No.:
P62805, P62806

Gene ID (Entrez):
121504

Immunogen:
Synthetic trimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot# P62805].

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration:
0.58 mg/mL

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Dot Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias:
H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4/p, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, H4K20Me3, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, histone 4, H4, histone cluster 4, H4, histone H4, MGC24116

Gene Symbols:
HIST4H4

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, mouse and rat. Predicted to react with most mammal species.

Img

Fischer Scientific

5073398

Rabbit Polyclonal Antibody has been...


Antigen:
Histone H4

Dilution:
Western Blot 2 ug/ml

Classification:
Polyclonal

Host Species:
Rabbit

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
121504

Immunogen:
This antibody was generated by immunizing rabbits with a synthetic peptide containing amino acid residues 15-30 of human Histone H4 GAKRHRKVLRDNIQGI. This sequence is conserved across mammalian, invertebrate and plant species. An NCBI BLAST shows no cross-reactivity of this sequence with other known histones.

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Concentration:
0.5 mg/mL

Applications:
Western Blot

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
PBS with 0.05% Sodium Azide

Gene Alias:
H4/A, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4/p, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, histone 4, H4, histone cluster 4, H4, histone H4, MGC24116

Gene Symbols:
HIST4H4

Isotype:
IgG

Purification Method:
Protein G purified

Test Specificity:
A 14 kDa band is observed. The has been evaluated in human, and most likely reacts with additional mammalian, invertebrate, and plant species as the immunogen sequence used to make it is conserved.

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5073500

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Antigen:
HMGB1/HMG-1

Dilution:
Western Blot 0.5-1.0 ug/ml, Simple Western 1:2000, Flow Cytometry 1:100, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 0.05 ug/ml, Immunohistochemistry-Paraffin 1:100-1:250, Flow (Intracellular)

Classification:
Polyclonal

Host Species:
Rabbit

Target Species:
Human, Mouse, Rat, Bovine, Canine, Rabbit, Sheep

Gene Accession No.:
P09429

Gene ID (Entrez):
3146

Immunogen:
A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C. Do not freeze.

Concentration:
1 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Tris-Glycine with 0.02% Sodium Azide

Gene Alias:
Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein

Gene Symbols:
HMGB1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, mouse, rat, dog, bovine, sheep. Predicted to react with porcine, equine and rabbit based on 100% sequence homology.

Img

Fischer Scientific

5073500

Rabbit Polyclonal Antibody has been...


Antigen:
HMGB1/HMG-1

Dilution:
Western Blot 0.5-1.0 ug/ml, Simple Western 1:2000, Flow Cytometry 1:100, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 0.05 ug/ml, Immunohistochemistry-Paraffin 1:100-1:250, Flow (Intracellular)

Classification:
Polyclonal

Host Species:
Rabbit

Target Species:
Human, Mouse, Rat, Bovine, Canine, Rabbit, Sheep

Gene Accession No.:
P09429

Gene ID (Entrez):
3146

Immunogen:
A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C. Do not freeze.

Concentration:
1 mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Formulation:
Tris-Glycine with 0.02% Sodium Azide

Gene Alias:
Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein

Gene Symbols:
HMGB1

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, mouse, rat, dog, bovine, sheep. Predicted to react with porcine, equine and rabbit based on 100% sequence homology.