5160670

CD3 Mouse anti-Human, BB515, Clone: HIT3a, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3

Applications

Flow Cytometry

Conjugate

Brilliant Blue 515 (BB515)

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

HIT3a

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Isotype

IgG2a κ

Purification Method

Affinity Purified

Description

CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916

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Description

  • The HIT3a monoclonal antibody specifically binds to the human CD3 ε-chain, a 20 kDa subunit of the CD3/T cell antigen receptor complex found on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
  • Studies from the HLDA Workshop show that this antibody can be mitogenic for T lymphocytes
  • The CD3 complex plays a role in signal transduction during antigen recognition by the T cell receptor
  • HIT3a antibody does not stain intracellular CD3 unlike the other CD3-specific clone, UCHT1 (Cat
  • No
  • 555330/550368)
  • The antibody was conjugated to BD Horizon BB515 which was developed exclusively by BD Biosciences
  • With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (eg, 530/30-nm filter)
  • This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Compare Similar Items

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Fischer Scientific

5160670

Mouse Monoclonal Antibody...


Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
Brilliant Blue 515 (BB515)

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
HIT3a

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
CD3E; T3E; TCRE; cd 3; cd-3; cd3; CD3-epsilon; 916

Img

Fischer Scientific

5160684

Mouse Monoclonal Antibody...


Antigen:
CD62L

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 711

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
SK11

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Cynomolgus, Human, Rhesus Monkey

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14

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Fischer Scientific

5160684

Mouse Monoclonal Antibody...


Antigen:
CD62L

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 711

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
SK11

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Cynomolgus, Human, Rhesus Monkey

Isotype:
IgG2a κ

Purification Method:
Affinity Purified

Description:
L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14

Img

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5160690

Mouse Monoclonal Antibody...


Antigen:
CD80

Applications:
Flow Cytometry, Immunohistochemistry, Western Blot

Conjugate:
Brilliant Blue 515 (BB515)

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
L307.4

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
B7.1; B7-1; Activation B7-1 antigen; B7; BB1; CD28LG; CD28LG1; LAB7