CD62L Mouse anti-Human, Brilliant Violet 711, Clone: SK11, BD

Description

• The SK11 monoclonal antibody specifically binds to CD62L which is also known as, L-selectin, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1/LECAM-1), Leukocyte adhesion molecule 1 (LAM1/LAM-1), Lymph node homing receptor (LNHR), Leu-8, or TQ1
• CD62L is an ∽80 kDa type I transmembrane glycoprotein that belongs to the Selectin/LECAM family
• CD62L is differentially expressed on T cells, B cells, monocytes, granulocytes, and a subset of NK cells
• The CD62L molecule is the human homolog of the mouse lymph node homing receptor, MEL-14
• CD62L plays a role in leucocyte binding to inflamed endothelium and extravasation, as well as mediating lymphocyte homing into peripheral lymphoid tissues through high endothelial postcapillary venules
• Soluble CD62L can result from the proteolytic cleavage of cell surface CD62L during cellular activation or inflammation
• The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.