CD36 Monoclonal Antibody (HM36), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII
• Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser CD36, also known as scavenger receptor class B member 3, is a protein that is expressed on the surface of various cell types, including macrophages, platelets, and adipocytes
• It plays a role in lipid metabolism, inflammation, and atherosclerosis, and is involved in the recognition and uptake of various ligands such as oxidized low-density lipoproteins, long-chain fatty acids, and apoptotic cells
• CD36 is also implicated in the pathogenesis of malaria
• The protein encoded by this gene serves as a receptor for thrombospondin in platelets and various cell lines, and is the fourth major glycoprotein of the platelet surface
• It binds to collagen, thrombospondin, anionic phospholipids, and oxidized LDL, and directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes
• Mutations in this gene cause platelet glycoprotein deficiency
• Multiple alternatively spliced transcript variants have been found for this gene
• Diseases associated with CD36 include Platelet Glycoprotein IV Deficiency and Coronary Heart Disease 7.