CD88 (C5aR1) Monoclonal Antibody (20/70), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This 20/70 monoclonal antibody recognizes mouse CD88 also known as C5aR1
• CD88 is a complement receptor widely expressed on cells of the myeloid lineage
• Clone 20/70 has been reported to block the binding of C5a to murine CD88
• Applications Reported: This 20/70 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 20/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 1.0 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known
• C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract
• CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a
• Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production
• CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex
• Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.