CD154 (CD40 Ligand) Monoclonal Antibody (MR1), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: The MR1 monoclonal antibody reacts with mouse CD154, a 39 kDa transmembrane glycoprotein also known as CD40 ligand (CD40L)
• CD154 is expressed transiently by activated T cells
• Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction
• CD154-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle
• For staining for flow cytometric analysis, it is important to stimulate enriched T cells or enriched CD4 cells (using depletion strategy) prior to staining with MR1
• Applications Reported: This MR1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This MR1 antibody has been tested by intracellular staining by flow cytometric analysis of stimulated mouse splenocytes
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells
• The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines
• CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes
• It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.