CD365 (Tim-1) Recombinant Rabbit Monoclonal Antibody (P365D), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This P365D recombinant rabbit monoclonal antibody reacts with human CD365, which is also known as Tim-1, Kim-1, or Havcr1
• Applications Tested: This P365D antibody has been pre-diluted and tested by flow cytometric analysis of 769P cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser Filtration: 0.2 μm post-manufacturing filtered
• CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail
• The endogenous ligand for CD365 is Tim-4
• In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus
• The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes
• Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity
• CD365 has also been implicated in the T cell co-stimulation
• CD365 is primarily expressed in epithelial cells
• It is also highly expressed in A549, 769-P and Caco-2 and cell lines
• CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.