CD366 (TIM3) Monoclonal Antibody (F38-2E2), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335
• CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail
• Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-)
• Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific
• Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains
• NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines
• The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro
• The 29A1.4 monoclonal antibody does not deplete NK cells in vivo
• Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells
• TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region
• TIM3 is involved in macrophage activation and induction of autoimmune diseases
• Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance
• Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.