53-979-282

GFAP Monoclonal Antibody (2.2B10), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Manufacturer: eBioscience

Select a Size

Pack Size SKU Availability Price
Each of 1 53-979-282-Each-of-1 In Stock ₹ 35,849.64

53-979-282 - Each of 1

₹ 35,849.64

In Stock

Quantity

1

Base Price: ₹ 35,849.64

GST (18%): ₹ 6,452.935

Total Price: ₹ 42,302.575

Antigen

GFAP

Classification

Monoclonal

Concentration

0.5 mg/mL

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.

P03995, P14136, P47819, Q28115

Gene Symbols

GFAP

Immunogen

Enriched bovine glial filaments

Quantity

100μg

Primary or Secondary

Primary

Target Species

Bovine, Human, Mouse, Rat

Product Type

Antibody

Isotype

IgG2a κ

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)

Clone

2.2B10

Conjugate

Alexa Fluor 488

Gene

GFAP

Gene Alias

6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen

Host Species

Rat

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

14580, 24387, 2670, 281189

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

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Description

  • Description: This 2.2B10 monoclonal antibody recognizes human, mouse, rat, and bovine glial fibrillary acid protein (GFAP)
  • The immunogen used to generate this antibody was enriched bovine glial filaments
  • Applications Reported: This 2.2B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
  • Applications Tested: This 2.2B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of U-251 cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol
  • Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
  • This may be used at less than or equal to 0.25 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family
  • GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves
  • In addition, neural stem cells strongly express GFAP
  • Antibodies to GFAP are very useful as markers of astrocytic cells
  • In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP
  • GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes
  • GFAP is used as a marker to distinguish astrocytes from other glial cells during development
  • Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system
  • Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

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eBioscience

53-979-282

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Antigen:
GFAP

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
P03995, P14136, P47819, Q28115

Gene Symbols:
GFAP

Immunogen:
Enriched bovine glial filaments

Quantity:
100μg

Primary or Secondary:
Primary

Target Species:
Bovine, Human, Mouse, Rat

Product Type:
Antibody

Isotype:
IgG2a κ

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen)

Clone:
2.2B10

Conjugate:
Alexa Fluor 488

Gene:
GFAP

Gene Alias:
6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen

Host Species:
Rat

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
14580, 24387, 2670, 281189

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Antigen:
O-GlcNAc

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
O15294

Gene Symbols:
OGT

Immunogen:
Rat liver nuclear envelopes

Quantity:
25 Tests

Primary or Secondary:
Primary

Target Species:
Chemical

Product Type:
Antibody

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
RL2

Conjugate:
Alexa Fluor 488

Gene:
OGT

Gene Alias:
GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase

Host Species:
Mouse

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
8473

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Fischer Scientific

53-979-342

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Antigen:
O-GlcNAc

Classification:
Monoclonal

Concentration:
5 μL/Test

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.:
O15294

Gene Symbols:
OGT

Immunogen:
Rat liver nuclear envelopes

Quantity:
100 Tests

Primary or Secondary:
Primary

Target Species:
Chemical

Product Type:
Antibody

Isotype:
IgG1 κ

Applications:
Flow Cytometry

Clone:
RL2

Conjugate:
Alexa Fluor 488

Gene:
OGT

Gene Alias:
GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; OGT; O-linked N-acetylglucosamine (GlcNAc) transferase; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase

Host Species:
Mouse

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
8473

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid

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Invitrogen

53-981-082

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Antigen:
Blood Group Antigen H (O) Type 1

Classification:
Monoclonal

Concentration:
0.5 mg/mL

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Accession No.:
__

Gene Symbols:
__

Immunogen:
__

Quantity:
100 μg

Primary or Secondary:
Primary

Target Species:
Human

Product Type:
Antibody

Isotype:
IgG3

Applications:
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Clone:
17-206

Conjugate:
Alexa Fluor 488

Gene:
__

Gene Alias:
ABH; BG-H; BG-H1; group h

Host Species:
Mouse

Purification Method:
Affinity chromatography

Regulatory Status:
RUO

Gene ID (Entrez):
__

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Form:
Liquid