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5323330

CD43/Sialophorin Antibody (DF-T1), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD43/Sialophorin

Concentration

0.2mg/mL

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

CD43 antigen, CD43), Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, sialophorin (gpL115, leukosialin, CD43)

Gene Symbols

SPN

Isotype

IgG1 κ

Purification Method

Protein G purified

Test Specificity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Clone

DF-T1

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

B Cell Development and Differentiation Markers, Immunology

Gene Accession No.

P16150

Gene ID (Entrez)

6693

Immunogen

Myeloblastic KG1 cells were used as the immunogen for this antibody.

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Description

  • CD43/Sialophorin Monoclonal specifically detects CD43/Sialophorin in Human samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Fischer Scientific

5323330

Mouse Monoclonal Antibody...

Antigen:
CD43/Sialophorin
Concentration:
0.2mg/mL
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CD43 antigen, CD43), Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, sialophorin (gpL115, leukosialin, CD43)
Gene Symbols:
SPN
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Clone:
DF-T1
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
B Cell Development and Differentiation Markers, Immunology
Gene Accession No.:
P16150
Gene ID (Entrez):
6693
Immunogen:
Myeloblastic KG1 cells were used as the immunogen for this antibody.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.

Img

Fischer Scientific

5323369

Rabbit Monoclonal Antibody...

Antigen:
PMEL17/SILV
Concentration:
0.5mg/mL
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA with 0.05% Sodium Azide
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols:
PMEL
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
__
Clone:
P14-V
Dilution:
Immunohistochemistry 1:100-1:200, Immunohistochemistry-Paraffin 1:100-1:200
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
__
Gene Accession No.:
__
Gene ID (Entrez):
6490
Immunogen:
Peptide derived from C-terminal sequence of human melanosome/gp10.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C. Do not freeze.

Img

Fischer Scientific

5323369

Rabbit Monoclonal Antibody...

Antigen:
PMEL17/SILV
Concentration:
0.5mg/mL
Classification:
Monoclonal
Form:
__
Regulatory Status:
RUO
Formulation:
20mM Tris/HCl (pH 8.0) and 20 mg/ml BSA with 0.05% Sodium Azide
Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17
Gene Symbols:
PMEL
Isotype:
__
Purification Method:
Affinity Purified
Test Specificity:
__
Clone:
P14-V
Dilution:
Immunohistochemistry 1:100-1:200, Immunohistochemistry-Paraffin 1:100-1:200
Conjugate:
Unconjugated
Host Species:
Rabbit
Research Discipline:
__
Gene Accession No.:
__
Gene ID (Entrez):
6490
Immunogen:
Peptide derived from C-terminal sequence of human melanosome/gp10.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C. Do not freeze.

Img

Fischer Scientific

5323454

Mouse Monoclonal Antibody...

Antigen:
Progesterone R/NR3C3
Concentration:
1.0 mg/mL
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS - BSA free with No Preservative
Gene Alias:
NR3C3PRNuclear receptor subfamily 3 group C member 3, progesterone receptor
Gene Symbols:
PGR
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kDa) and PR-B (116kDa). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues.
Clone:
PR484
Dilution:
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, CyTOF-ready
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Breast Cancer, Cancer
Gene Accession No.:
__
Gene ID (Entrez):
5241
Immunogen:
A progesterone receptor recombinant protein was used as the immunogen for the antibody. The specific epitope has not been mapped.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.