5349709

MilliporeSigma™ anti-Ly-6G FITC, Clone: RB6-8C5,

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Ly-6G

Applications

Flow Cytometry

Conjugate

FITC

Host Species

Rat

Research Discipline

Inflammation, Immunology

Formulation

Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols

Ly6g

Primary or Secondary

Primary

Content And Storage

2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

Clone

RB6-8C5

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Mouse

Gene Accession No.

P35461

Immunogen

Proprietary immunogen

Purification Method

Purified by size-exclusion chromatography

Description

  • Specifically detects Ly-6G Clone: RB6-8C5 in Mouse samples, and it is validated for Flow Cytometry The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion
  • Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils
  • In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al
  • 1993
  • J
  • Immunol
  • 151:2399-2408 and Sasmono et al
  • 2007
  • J
  • Leukoc
  • Biol
  • 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C
  • Other reports suggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S
  • and Schlueter AJ
  • 2003
  • Cytometry A, 58(2): 195-200.

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Rat Monoclonal Antibody...


Antigen:
Ly-6G

Applications:
Flow Cytometry

Conjugate:
FITC

Host Species:
Rat

Research Discipline:
Inflammation, Immunology

Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols:
Ly6g

Primary or Secondary:
Primary

Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze

Clone:
RB6-8C5

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Accession No.:
P35461

Immunogen:
Proprietary immunogen

Purification Method:
Purified by size-exclusion chromatography

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Flow Cytometry

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Inflammation, Immunology

Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols:
Ly6g

Primary or Secondary:
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Content And Storage:
2°C to 8°C for one year from date of shipment protected from light; Do not freeze

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Purified by size-exclusion chromatography

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Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols:
Ly6g

Primary or Secondary:
Primary

Content And Storage:
2°C to 8°C for one year from date of shipment protected from light; Do not freeze

Clone:
1A8

Classification:
Monoclonal

Form:
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RUO

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Mouse

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P35461

Immunogen:
Proprietary immunogen

Purification Method:
Purified by size-exclusion chromatography

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Ly-6G

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Flow Cytometry

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Inflammation, Immunology

Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.

Gene Symbols:
Ly6g

Primary or Secondary:
Primary

Content And Storage:
2°C to 8°C for one year from date of shipment protected from light; Do not freeze

Clone:
1A8

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Mouse

Gene Accession No.:
P35461

Immunogen:
Proprietary immunogen

Purification Method:
Purified by size-exclusion chromatography