5539197

CD3 epsilon Antibody (B-B12), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD3 epsilon

Concentration

0.2 mg/mL

Applications

Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Adaptive Immunity, Apoptosis, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Lines, Stem Cell Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez)

916

Immunogen

Human T cell leukemia cells

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

20 kDa

Clone

B-B12

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE

Gene Symbols

CD3E

Isotype

IgG1 κ

Purification Method

Protein A purified

Test Specificity

Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence CD3 epsilon Monoclonal specifically detects CD3 epsilon in Human samples
  • It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

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Img

Fischer Scientific

5539197

Mouse Monoclonal Antibody...


Antigen:
CD3 epsilon

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Apoptosis, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mesenchymal Stem Cell Markers, Signal Transduction, Stem Cell Lines, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
916

Immunogen:
Human T cell leukemia cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
20 kDa

Clone:
B-B12

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, FLJ18683, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE

Gene Symbols:
CD3E

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Reacts with five invariable CD3 chains (designated as and ) with molecular weight ranging from 16-28kDa. CD3 is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. The CD3 complex is closely associated at the lymphocyte cell surface with T cell antigen receptor (TCR) and is involved in transducing antigen-recognition signals into cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.

Img

Fischer Scientific

5539210

Mouse Monoclonal Antibody...


Antigen:
CD4

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Immunology, Innate Immunity, Regulatory Immunology, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
920

Immunogen:
Recombinant human CD4 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
55 kDa

Clone:
C4/206

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene Symbols:
__

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 55kDa, identified as CD4. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified

Img

Fischer Scientific

5539210

Mouse Monoclonal Antibody...


Antigen:
CD4

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Immunology, Innate Immunity, Regulatory Immunology, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
920

Immunogen:
Recombinant human CD4 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
55 kDa

Clone:
C4/206

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene Symbols:
__

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 55kDa, identified as CD4. CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified

Img

Fischer Scientific

5539211

Mouse Monoclonal Antibody...


Antigen:
CD4

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Immunology, Innate Immunity, Regulatory Immunology, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
920

Immunogen:
Stimulated human leukocytes

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
55 kDa

Clone:
EDU-2

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Monkey, Orangutan

Gene Alias:
CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene Symbols:
__

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. This MAb was characterized as human CD4 antibody at II and IV International Workshop on Human Leukocyte Differentiation Antigens.