5539554

CD45RB Antibody (PTPRC/1132), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD45RB

Concentration

0.2mg/mL

Applications

Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols

PTPRC

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Clone

PTPRC/1132

Dilution

Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Monkey (Negative), Rat (Negative)

Gene Accession No.

P08575

Gene ID (Entrez)

5788

Immunogen

Recombinant full-length human CD45RB protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

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Description

  • Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence CD45RB Monoclonal specifically detects CD45RB in Human, Monkey (Negative), Rat (Negative) samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Compare Similar Items

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Fischer Scientific

5539554

Mouse Monoclonal Antibody...


Antigen:
CD45RB

Concentration:
0.2mg/mL

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols:
PTPRC

Isotype:
IgG1 κ

Purification Method:
Protein A or G purified

Test Specificity:
CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.

Clone:
PTPRC/1132

Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Monkey (Negative), Rat (Negative)

Gene Accession No.:
P08575

Gene ID (Entrez):
5788

Immunogen:
Recombinant full-length human CD45RB protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Img

Fischer Scientific

5539556

Mouse Monoclonal Antibody...


Antigen:
CD45RO

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols:
PTPRC

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Clone:
190-2F2.5

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P08575

Gene ID (Entrez):
5788

Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell).

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Img

Fischer Scientific

5539556

Mouse Monoclonal Antibody...


Antigen:
CD45RO

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols:
PTPRC

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Clone:
190-2F2.5

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
P08575

Gene ID (Entrez):
5788

Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell).

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Img

Fischer Scientific

5539557

Mouse Monoclonal Antibody...


Antigen:
CD45RO

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols:
PTPRC

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Clone:
T200/797

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
__

Gene ID (Entrez):
5788

Immunogen:
Recombinant human CD45RO protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.