5539550
CD45RA Antibody (PTPRC/1148), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD45RA
Concentration
0.2mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez)
5788
Immunogen
Recombinant full-length human CD45RA protein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Clone
PTPRC/1148
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols
PTPRC
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
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Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence CD45RA Monoclonal specifically detects CD45RA in Human samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.
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Antigen: CD45RA
Concentration: 0.2mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 5788
Immunogen: Recombinant full-length human CD45RA protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: PTPRC/1148
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols: PTPRC
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Antigen:
CD45RA
Concentration:
0.2mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
5788
Immunogen:
Recombinant full-length human CD45RA protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
PTPRC/1148
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols:
PTPRC
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Antigen: CD45RB
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 5788
Immunogen: Recombinant full-length human CD45RB protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: PTPRC/1132
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Monkey (Negative), Rat (Negative)
Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols: PTPRC
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Antigen:
CD45RB
Concentration:
0.2mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
5788
Immunogen:
Recombinant full-length human CD45RB protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
PTPRC/1132
Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Monkey (Negative), Rat (Negative)
Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols:
PTPRC
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
CD45R, also designated CD45 and PTPRC, is identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin.
Antigen: CD45RO
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 5788
Immunogen: Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell).
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: 190-2F2.5
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols: PTPRC
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Antigen:
CD45RO
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
5788
Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
190-2F2.5
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols:
PTPRC
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Antigen: CD45RO
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, SDS-Page, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 5788
Immunogen: Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell).
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: 190-2F2.5
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols: PTPRC
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Antigen:
CD45RO
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, SDS-Page, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
5788
Immunogen:
Lentillectin purified glycoproteins obtained from HuPBL and from a CLL (B cell).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
190-2F2.5
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols:
PTPRC
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.