5539558

CD45RO Antibody (UCHL-1 + T200/797), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD45RO

Concentration

0.2mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation

10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez)

5788

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1); Recombinant human CD45RO protein (T200/797)

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Clone

UCHL-1 + T200/797

Dilution

Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols

PTPRC

Isotype

IgG

Purification Method

Protein A or G purified

Test Specificity

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

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Description

  • Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence CD45RO Monoclonal specifically detects CD45RO in Human samples
  • It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

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Fischer Scientific

5539558

Mouse Monoclonal Antibody...


Antigen:
CD45RO

Concentration:
0.2mg/mL

Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Adaptive Immunity, Cell Biology, Cellular Markers, Cytokine Research, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Microglia Markers, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
5788

Immunogen:
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1); Recombinant human CD45RO protein (T200/797)

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
UCHL-1 + T200/797

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbols:
PTPRC

Isotype:
IgG

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

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Antigen:
CD46

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Immunology

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
4179

Immunogen:
Stimulated human leukocytes

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
197-2B1

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
AHUS2, CD46 antigen, CD46 molecule, complement regulatory protein, membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen), MIC10, TLXcomplement regulatory protein, trophoblast leucocyte common antigen, Trophoblast leukocyte common antigen, trophoblast-lymphocyte cross-reactive antigen

Gene Symbols:
CD46

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Recognizes a protein of 52kDa-58kDa, identified as CD46 (also known as membrane cofactor protein, MCP). CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria. This MAb can be applied to test complement activation in pseudo-allergic reactions to acetylsalicylic acid and to test for measles virus infection of cells.

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Antigen:
CD48/SLAMF2

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cell Biology, Cellular Markers, Immunology, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
962

Immunogen:
Human peripheral blood lymphocytes

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
5-4.8

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
BCM1 surface antigen, BCM1Leukocyte antigen MEM-102, BLAST, BLAST1TCT.1, B-lymphocyte activation marker BLAST-1, CD48 antigen, CD48 antigen (B-cell membrane protein), CD48 molecule, hCD48, mCD48, MEM-102, SLAMF2

Gene Symbols:
CD48

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.

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Antigen:
CD48/SLAMF2

Concentration:
0.2 mg/mL

Applications:
Flow Cytometry, SDS-Page, Immunofluorescence

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cell Biology, Cellular Markers, Immunology, Stem Cell Markers

Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide

Gene ID (Entrez):
962

Immunogen:
Human peripheral blood lymphocytes

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Clone:
5-4.8

Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Alias:
BCM1 surface antigen, BCM1Leukocyte antigen MEM-102, BLAST, BLAST1TCT.1, B-lymphocyte activation marker BLAST-1, CD48 antigen, CD48 antigen (B-cell membrane protein), CD48 molecule, hCD48, mCD48, MEM-102, SLAMF2

Gene Symbols:
CD48

Isotype:
IgG2a κ

Purification Method:
Protein A or G purified

Test Specificity:
Reacts with human CD48, a 45kDa glycosyl phophatidyl-inositol (GPI)-anchored cell surface protein. CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2.