EpCAM/TROP1 Antibody (MOC-31), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 3 publications
Manufacturer: Fischer Scientific
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Antigen
EpCAM/TROP1
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cancer Stem Cells
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
Gene Symbols
EPCAM
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Clone
MOC-31
Dilution
Western Blot 1:100 - 1:200, Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100 - 1:200, SDS-Page, Immunofluorescence 1:50 - 1:100
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Rat (Negative)
Gene Accession No.
P16422
Gene ID (Entrez)
4072
Immunogen
Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
41 kDa
Related Products
Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence EpCAM/TROP1 Monoclonal specifically detects EpCAM/TROP1 in Human, Rat (Negative) samples
- It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: EpCAM/TROP1
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cancer Stem Cells
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: 17-1A, 323/A3, ACSTD1, antigen identified by monoclonal AUA1, CD326 antigen, Cell surface glycoprotein Trop-1, chromosome 4, surface marker (35kD glycoprotein), DIAR5, EGP, EGP-2, EGP314, EGP40, EpCAM, epithelial cell adhesion molecule, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, ESA, GA733-2EGP34, hEGP314, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1Ly74, Major gastrointestinal tumor-associated protein GA733-2, MIC18MH99, MOC31, TACST-1, TACSTD1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A
Gene Symbols: EPCAM
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Clone: MOC-31
Dilution: Western Blot 1:100 - 1:200, Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100 - 1:200, SDS-Page, Immunofluorescence 1:50 - 1:100
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Rat (Negative)
Gene Accession No.: P16422
Gene ID (Entrez): 4072
Immunogen: Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 41 kDa
Antigen: __
Concentration: __
Applications: __
Conjugate: __
Host Species: __
Research Discipline: __
Formulation: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Test Specificity: __
Clone: __
Dilution: __
Classification: __
Form: __
Regulatory Status: __
Target Species: __
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
Molecular Weight of Antigen: __
Antigen: Neprilysin/CD10
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cell Biology, Cytokine Research, Immunology, Stem Cell Markers
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: Atriopeptidase, CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase), CD10 antigen, CD10), CD10membrane metallo-endopeptidase variant 1, Common acute lymphocytic leukemia antigen, DKFZp686O16152, EC 3.4.24, EC 3.4.24.11, Enkephalinase, EPN, membrane metallo-endopeptidase, membrane metallo-endopeptidase variant 2, MGC126681, MGC126707, NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11, SFE, Skin fibroblast elastase
Gene Symbols: MME
Isotype: IgG2b κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Clone: CB-CALLA
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P08473
Gene ID (Entrez): 4311
Immunogen: Human CD10
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 100 kDa
