Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Golgi Glycoprotein 1/GLG1
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human, Mouse (Negative), Rat (Negative)
Gene Accession No.
Q92896, Q92896
Gene ID (Entrez)
2734
Immunogen
Golgi fraction from human liver cells
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
134 kDa
Clone
GLG1/970
Dilution
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias
CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
Gene Symbols
GLG1
Isotype
IgG1 κ
Purification Method
Protein A or G purified
Test Specificity
This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three fun
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Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence Golgi Glycoprotein 1/GLG1 Monoclonal specifically detects Golgi Glycoprotein 1/GLG1 in Human, Mouse (Negative), Rat (Negative) samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.
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Antigen: Golgi Glycoprotein 1/GLG1
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Mouse (Negative), Rat (Negative)
Gene Accession No.: Q92896, Q92896
Gene ID (Entrez): 2734
Immunogen: Golgi fraction from human liver cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 134 kDa
Clone: GLG1/970
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
Gene Symbols: GLG1
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three fun
Antigen: __
Concentration: __
Applications: __
Conjugate: __
Host Species: __
Target Species: __
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
Molecular Weight of Antigen: __
Clone: __
Dilution: __
Classification: __
Form: __
Regulatory Status: __
Formulation: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Test Specificity: __
Antigen: gp96/HSP90B1/GRP94
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Rat
Target Species: Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Accession No.: __
Gene ID (Entrez): 7184
Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 94 kDa
Clone: 9G10.F8.2
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
Gene Symbols: HSP90B1
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
