5540659
gp96/HSP90B1/GRP94 Antibody (SPM249), Novus Biologicals™
Rat Monoclonal Antibody has been used in 1 publication
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
gp96/HSP90B1/GRP94
Concentration
0.2mg/mL
Applications
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate
Unconjugated
Host Species
Rat
Research Discipline
Cancer, Cellular Markers, ER Markers, Golgi Apparatus Markers, Hypoxia, Membrane Trafficking and Chaperones
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez)
7184
Immunogen
Purified glucose regulated protein 94 (grp94) from chicken oviducts
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
94 kDa
Clone
SPM249
Dilution
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Alias
94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
Gene Symbols
HSP90B1
Isotype
IgG2a κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
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Description
- Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence gp96/HSP90B1/GRP94 Monoclonal specifically detects gp96/HSP90B1/GRP94 in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: gp96/HSP90B1/GRP94
Concentration: 0.2mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Rat
Research Discipline: Cancer, Cellular Markers, ER Markers, Golgi Apparatus Markers, Hypoxia, Membrane Trafficking and Chaperones
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 7184
Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 94 kDa
Clone: SPM249
Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Alias: 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
Gene Symbols: HSP90B1
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Antigen:
gp96/HSP90B1/GRP94
Concentration:
0.2mg/mL
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Rat
Research Discipline:
Cancer, Cellular Markers, ER Markers, Golgi Apparatus Markers, Hypoxia, Membrane Trafficking and Chaperones
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
7184
Immunogen:
Purified glucose regulated protein 94 (grp94) from chicken oviducts
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
94 kDa
Clone:
SPM249
Dilution:
Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Porcine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Alias:
94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
Gene Symbols:
HSP90B1
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
Antigen: Histone H1
Concentration: 0.2mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 3024
Immunogen: Recombinant full-length human Histone H1 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: HH1/957
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat
Gene Alias: H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345
Gene Symbols: H1
Isotype: IgG2a κ
Purification Method: Protein A or G purified
Test Specificity: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Antigen:
Histone H1
Concentration:
0.2mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
3024
Immunogen:
Recombinant full-length human Histone H1 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__
Clone:
HH1/957
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat
Gene Alias:
H1 histone family, member 1, H1.1, H1A, H1F1HIST1, histone 1, H1a, histone cluster 1, H1a, histone H1.1, MGC126642, MGC138345
Gene Symbols:
H1
Isotype:
IgG2a κ
Purification Method:
Protein A or G purified
Test Specificity:
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Antigen: Helicobacter Pylori
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): __
Immunogen: Total sonicate of Helicobacter pylori
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: B-53.4
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 1 - 2 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: H. pylori
Gene Symbols: __
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Antigen:
Helicobacter Pylori
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
__
Immunogen:
Total sonicate of Helicobacter pylori
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__
Clone:
B-53.4
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 1 - 2 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
H. pylori
Gene Symbols:
__
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Antigen: Helicobacter Pylori
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: __
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): __
Immunogen: Total sonicate of Helicobacter pylori
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: HP/212
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 1 - 2 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Bacteria
Gene Alias: H. pylori
Gene Symbols: __
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Antigen:
Helicobacter Pylori
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
__
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
__
Immunogen:
Total sonicate of Helicobacter pylori
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__
Clone:
HP/212
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 1 - 2 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Bacteria
Gene Alias:
H. pylori
Gene Symbols:
__
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
The spiral shaped bacterium Helicobacter pyloriis strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pyloriis known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual H. pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.